Mouldy Sioud
Combination of Chemical and Enzymatic RNA Synthesis
Rajesh K. Gaur, Andreas Hanne, and Guido Krupp
Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes
Martha J. Fedor
Real-Time Monitoring of RNA and DNA Reactions by Fluorescence Detection
Kumud K. Singh, Andreas Hanne, and Guido Krupp
Quantification of Ribozyme Target RNA Using Real-Time PCR
Dagmar Klein, Camillo Ricordi, and Ricardo L. Pastori
Analysis of Ribozyme Structure and Function by Nucleotide Analog Interference Mapping
Soumitra Basu, Catherine Pazsint, and Gopeswar Chowdhury
Analysis of Global Conformational Transitions in Ribozymes
David M. J. Lilley
In Vivo Detection of Ribozyme Cleavage Products and RNA Structure by Use of Terminal Transferase-Dependent PCR
Hsiu-Hua Chen, Daniela Castanotto, Jeanne LeBon, John J. Rossi, and Arthur D. Riggs
Identification of Efficient Cleavage Sites in Long-Target RNAs
Wei-Hua Pan and Gary A. Clawson
Selection In Vitro of Allosteric Ribozymes
Adam Roth and Ronald R. Breaker
Regulation of Ribozyme Cleavage Activity by Oligonucleotides
Yasuo Komatsu and Eiko Ohtsuka
Tetracycline-Regulated Expression of Hammerhead Ribozymes In Vivo
Emma T. Bowden and Anna T. Riegel
Ribozyme Expression Systems
Masayuki Sano and Kazunari Taira
Design and Expression of Chimeric U1/Ribozyme Transgenes
Roger Abounader, Robert Montgomery, Harry Dietz, and John Laterra
Design and Validation of Therapeutic Hammerhead Ribozymes for Autosomal Dominant Diseases
Jason J. Fritz, Marina Gorbatyuk, Alfred S. Lewin, and William W. Hauswirth
Helicase-Attached Novel Hybrid Ribozymes
Hiroaki Kawasaki, Masaki Warashina, Tomoko Kuwabara, and Kazunari Taira
Functional Gene Discovery Using Hybrid Ribozyme Libraries
Yoshio Kato, Masaru Tsunemi, Makoto Miyagishi, Hiroaki Kawasaki, and Kazunari Taira
Maxizyme Technology
Mayu Iyo, Hiroaki Kawasaki, and Kazunari Taira
Target-Site Selection for the 10–23 DNAzyme
Murray J. Cairns and Lun-Quan Sun
In Vitro Selected RNA-Cleaving DNA Enzymes From Combinatorial Libraries
Samitabh Chakraborti, Bandi Sriram and Akhil C. Banerjea
Nucleic Acid Sequence Analysis Using DNAzymes
Murray J. Cairns and Lun-Quan Sun
Crystallization of the Hairpin Ribozyme: Illustrative Protocols
Peter B. Rupert and Adrian R. Ferré-D’Amaré
An Experimental Method for Selecting Effective Target Sites and Designing Hairpin Ribozymes
Alicia Barroso-delJesus, Elena Puerta-Fernández, Cristina Romero-López, and Alfredo Berzal-Herranz
Design and Optimization of Sequence-Specific Hairpin Ribozymes
Cristina Romero-López, Alicia Barroso-delJesus, Elena Puerta-Fernández, and Alfredo Berzal-Herranz
Design, Targeting, and Initial Screening of sTRSV-Derived Hairpin Ribozymes for Optimum Helix 1 Length and Catalytic Efficiency In Vitro
Max W. Richardson, Linda Hostalek, Michelle Dobson, Jason Hu, Richard Shippy, Andrew Siwkowski, Jonathan D. Marmur, Kamel Khalili, Paul E. Klotman, Arnold Hampel, and Jay Rappaport
Optimization and Application of the Group I Ribozyme Trans-Splicing Reaction
Christer Einvik, Tonje Fiskaa, Eirik W. Lundblad, and Steinar Johansen
Repair of Myotonic Dystrophy Protein Kinase (DMPK) Transcripts by Trans-Splicing Ribozymes
Leonidas A. Phylactou
General Design and Construction of RNase P Ribozymes for Gene-Targeting Application
Hua Zou, Karen Chan, Phong Trang, and Fenyong Liu
In Vitro Selection of RNase P Ribozymes That Efficiently Cleave a Target mRNA
Kihoon Kim and Fenyong Liu
In Vitro Selection of External Guide Sequences for Directing Human RNase P to Cleave a Target mRNA
Stephen Raj and Fenyong Liu
RNase P-Mediated Inhibition of Viral Growth by Exogenous Administration of Short Oligonucleotide External Guide Sequence
Walter Dunn and Fenyong Liu
RNase P Ribozyme As an Antiviral Agent Against Human Cytomegalovirus
Phong Trang and Fenyong Liu
Inhibition of Gene Expression by Nucleic Acid Enzymes in Rodent Models of Human Disease
Per Ole Iversen and Mouldy Sioud
Potential Design Rules and Enzymatic Synthesis of siRNAs
Mouldy Sioud and Marianne Leirdal
RNA Interference (RNAi) With RNase III-Prepared siRNAs
Dun Yang, Andrei Goga, and J. Michael Bishop
RNAi Expression Vectors in Mammalian Cells
Makoto Miyagishi and Kazunari Taira
Gene-Array Analysis of Glioma Cells After Treatment With Anti-PKC-a siRNA
Marianne Leirdal and Mouldy Sioud
RNAi in Living Mice
Hidetoshi Hasuwa and Masaru Okabe
Construction and Transfection of PCR Products Expressing siRNAs or shRNAs in Mammalian Cells
Daniela Castanotto and John J. Rossi
Systemic Delivery of siRNAs
Mouldy Sioud and Dag R. Sorensen
Adenovirus-Delivered siRNA
Changxian Shen and Sven N. Reske
RNAi Expression Vectors in Plant Cells
Hideo Akashi, Makoto Miyagishi, and Kazunari Taira
Delivery Agents for Oligonucleotides
Olivier Seksek and Jacques Bolard
Selection of Peptides for Specific Delivery of Oligonucleotides Into Cancer Cells
Mohsen Shadidi and Mouldy Sioud
Clinical Gene Therapy Research Utilizing Ribozymes: Application to the Treatment of HIV/AIDS
Frances K. Ngok, Ronald T. Mitsuyasu, Janet L. Macpherson, Maureen P. Boyd, Geoff P. Symonds, and Rafael G. Amado
Critical Steps in the Implementation of Hematopoietic Progenitor-Cell Gene Therapy Using Ribozyme Vectors: A Laboratory Protocol
Maureen P. Boyd, Frances K. Ngok, Alison V. Todd, Geoff P. Symonds, Janet L. Macpherson, and Rafael G. Amado
