Michael-Christopher Keogh and Stephen Buratowski
Identifying PhosphoCTD-Associating Proteins
Hemali P. Phatnani and Arno L. Greenleaf
Imaging Alternative Splicing in Living Cells
Eric J. Wagner, Andrea Baines, Todd Albrecht, Robert M. Brazas, and Mariano A. Garcia-Blanco
Tobramycin Affinity Tag Purification of Spliceosomes
Klaus Hartmuth, Hans-Peter Vornlocher, and Reinhard Lührmann
Using Single-Strand Conformational Polymorphism Gel Electrophoresis to Analyze Mutually Exclusive Alternative Splicing
Alicia M. Celotto and Brenton R. Graveley
Methods for the Analysis of Adenosine-to-Inosine Editing in RNA
Zuo Zhang and Gordon G. Carmichael
Assaying Nuclear Messenger RNA Export in Human Cells
Bryan R. Cullen
RNA Unwinding Assay for DExD/H-Box RNA Helicases
Stephanie S. Tseng-Rogenski and Tien-Hsien Chang
Approaches for Monitoring Nuclear Translation
Francisco J. Iborra, Dean A. Jackson, and Peter R. Cook
Immunopurification and Analysis of Protein and RNA Components of mRNP in Mammalian Cells
Fabrice Lejeune and Lynne E. Maquat
Gene Expression Analysis of Messenger RNP Complexes
Luiz O. F. Penalva, Scott A. Tenenbaum, and Jack D. Keene
Using the lN Peptide to Tether Proteins to RNAs
Julie Baron-Benhamou, Niels H. Gehring, Andreas E. Kulozik, and Matthias W. Hentze
An Efficient System for Cap- and Poly(A)-Dependent Translation In Vitro
Yuri V. Svitkin and Nahum Sonenberg
A Poly(A) Tail-Responsive In Vitro System for Cap- or IRES-Driven Translation From HeLa Cells
Christian Thoma, Antje Ostareck-Lederer, and Matthias W. Hentze
Assessing Messenger RNA Decapping in Cellular Extracts
Naomi Bergman, Joseph Milone, Elizabeth J. Bates, Mateusz Opyrchal, Vivian Bellofatto, and Jeffrey Wilusz
Analysis of RNA Exonucleolytic Activities in Cellular Extracts
Devi Mukherjee, David T. Fritz, Walter J. Kilpatrick, Min Gao, and Jeffrey Wilusz
Application of Ligation-Mediated Reverse Transcription Polymerase Chain Reaction to the Identification of In Vivo Endonuclease-Generated Messenger RNA Decay Intermediates
Mark N. Hanson and Daniel R. Schoenberg
Biochemical Dissection of RNA Silencing in Plants
Guiliang Tang and Phillip D. Zamore
RNA Interference of mRNA Processing Factors in Drosophila S2 Cells
Alicia M. Celotto and Brenton R. Graveley
RNA Interference by Short Hairpin RNAs Expressed in Vertebrate Cells
Gregory J. Hannon and Douglas S. Conklin
Index
