Cloning Enzymes, one in the Enzyme Resource Guide series, highlights those enzymes important in nucleic acid cloning procedures. Enzymes that modify nucleic acids provide the foundation for many molecular biology techniques: enzymes that are used to synthesize, degrade, join or remove portions of nucleic acids in a controlled and generally defined manner. Specific features of the in vivo functions of these enzymes have been exploited in vitro to provide many of the protocols currently used in nucleic acid manipulations.This guide mainly is targeted Ligases, Kinases and Phosphatases.

Table of Contents Page

Enzyme Activities Diagram
The Cloning Enzymes
Gene Cloning
Ligases
Kinases and Phosphatases
RecA Protein and AgarACE® Enzyme
Technical Appendix