一款小巧的DNA分析软件
为了确定蛋白家族新成员的基因序列，常常需要根据蛋白序列对比设计核苷酸序列。 DNAPROBE便是用来帮助在这种情况下进行核苷酸序列设计。
绿色软件免安装。
To isolate genes for new members of a protein family, it is frequently necessary to design oligonucleotides on the basis of protein alignments.DNAPROBE assists in the design of oligonucleotides in such cases. It uses MSF protein alignments generated by the program PILEUP and codon frequency tables from the Wisconsin (GCG) sequence analysis software package. The basic algorithm calculates the most probable nucleotide sequence corresponding to the alignment by summing the products of the frequency with which a particular amino acid occurs at any one position and the codon usage frequency for that amino acid. Where an alignment contains a cluster of closely related sequences, the user may choose to implement a weighting option that reduces their individual contributions to the output in order that they do not overwhelm the contributions of more distantly related sequences which are under-represented. This may be done in two ways; (a) using the algorithm of Gerstein et al. (J.Mol. Biol., 236, 1067-1078) and the information contained in the dendrogram file written by PILEUP, or (b) by specifying weights in a separate file which is then read by the program, an option which enables one to give increased weight to sequences known to be more closely related to the target organism.
