由加拿大的Premier公司开发的专业用于PCR或测序引物以及杂交探针的设计和评估的软件，和Plasmid Premier2.02一起是该公司推出的最新的软件产品。其主要界面同样也是分为序列编辑窗口（Genetank），引物设计窗口（Primer Design），酶切分析窗口（Restriction Sites）和Motif分析窗口。这里我们主要介绍其引物设计功能，顾名思义，该软件就是用来进行引物设计的。可以简单地通过手动拖动鼠标以扩增出相应片段所需的引物，而在手动的任何时候，下面显示各种参数的改变和可能的二聚体、异二聚体、发夹结构等。也可以给定条件，让软件自动搜索引物，并将引物分析结果显示出来。而且进行这些操作非常简单。其功能绝不在Oligo 5.0之下！
详细使用帮助：http://www.bbioo.com/bbs/thread-14759-1-1.html
Features
Sequence Editor
Long PCR- Handles sequences up to 50 KB.
Alternate views- View sequence as sense, anti-sense, or double stranded.
Importing sequences- Complimenting or reversing sequence while cut, copy, paste.
Voice readback- Multimedia readback of sequences.
Translations- Translate and reverse-translate between DNA and protein sequences, easy switching between the original and translated sequences.
Edit codon table- Add new codon tables or choose from the universal or comprehensive set of mitochondria codon tables.
Find- Finds occurrences of specified sequence.
Grouping- Displays bases in groups of 3 or 10.
Restriction Enzyme Analysis
Enzyme filtering- Filter enzymes based on overhang sequence, recognition site length, or heat inactivation.
Enzyme editing- Adds and edits custom enzymes.
Motif Analysis
Motif analysis- Scan the sequence for nucleic acid motifs.
Motif editing- Edits already existing motifs or adds new ones.
Primer Design
PCR/ Hybridization/Sequencing primer search- Locates optimal primers for PCR, hybridization, or sequencing.
Automatic search- automatic search automatically relaxes stringency to locate the best primers available on the sequence.
Degenerate Primer Design- Reverse translate a protein sequence and design primers in regions of low degeneracy. Based on our research published in: Vinay Singh, Sita Naik, “A Program for Design of Degenerate Primers from a Protein Sequence”, Biotechniques, February 1998.
Manual Search- Allows researcher to set specific primer properties and locates all primers with these properties.
Secondary structures- Analyzes primers for hairpins, self-dimers, cross-dimers, and false priming sites.
Multiplex/Nested primers- Check primer pools and preexisting primers for cross homologies.
False priming- Check primers against a directory of sequences for false priming.
Site directed mutagenesis- Easily incorporate enzyme sites and silent mutations.
Analyze existing primers- Paste in any primer sequence for analysis, check for its binding sites on sequence.
Primer rating- Ranks both individual primers and primer pairs based on priming efficiency.
Comprehensive primer report- Prints comprehensive report for pairs and individual primers.
Printing of all secondary structures- Print all secondary structures for a primer.
Primer Database
Primer databases- Store multiple sets of primers with easy primer ordering.
Order forms- Easily order primers using customizable order form.
Printing Features
Comprehensive primer report- Prints comprehensive report for pairs and individual primers.
Multiplex/nested primers- Prints searched and selected primers over the sequence symbolically and selected primers in tabular format along with their cross dimers.
Printing of all secondary structures- Print all primer secondary structures.
Other Features
Hybridization time- Predict hybridization time of probes.
Optical activity- Calculates optical activity of primer in nmol/A260 and g/A260.
Molecular weight- Calculates primer molecular weight.
Pasting oligonucleotides- Oligonucleotides can be pasted as is, reversed, complemented or reverse complemented.
